Peptide Dilutent

Peptide Reconstitution Guidelines

How to Reconstitute (mix) a Peptide

Generally, it is advised to first attempt to dissolve peptides in solvents that are easy to remove by lyophilization. This is a precaution: in case the initial solvent is not effective, it can be removed again by the lyophilization process. Typically, the researcher should first attempt to dissolve the peptide in sterile distilled water or regular bacteriostatic water or in sterile dilute acetic acid (0.1%) solution. As a general guideline, it is recommended to test a small portion of the peptide for solubility in the chosen solvent before attempting to dissolve the entire peptide.

Importantly, the initial use of sterile water (or dilute acetic acid) will allow the researcher to dry the peptide without any unwanted residues in case the peptide fails to dissolve. Once the initial ineffective solvent is removed, the researcher can then attempt to dissolve the peptide in increasingly stronger solvents.

Additionally, researchers should dissolve the peptide in a sterile solvent to give a stock solution that is at a higher concentration than required for the assay. If the assay buffer is used first and the peptide does not dissolve, it can be very difficult to recover the peptide unadulterated. However, the peptide can always be diluted further with the assay buffer later on.


Sonication

In the laboratory, sonication can be tried as method to improve the rate of peptide dissolution in the solvent if the peptide continues to persist as visible particles in the solution. Sonication will not change the peptide’s solubility characteristics in a given solvent; the sonication process merely assists with breaking down lumps of solid peptide and briskly stirring the solution. After the sonication process, the researcher should examine the solution to see if it is cloudy, has gelled, or has any type of surface scum. If so, it is likely that the peptide is only suspended in the solution, not dissolved; therefore, a stronger solvent will probably be required.

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BACTERIOSTATIC WATER 0.9%
PRODUCT INFO Item: 50 Is a sterile, nonpyrogenic preparation of water for injection containing 0.9% (9 mg/mL) of benzyl alcohol added as a bacteriostatic preservative. Bacteriostatic water can be used for multiple injections spread over up 28 days. Bacteriostatic water is sterile water which contains a bacteriostatic preservative. 10 ml glass vial/container. Effective dosage for most ranges from 1ml - 2ml for every vial peptides.
$19.00
ACETIC ACID 0.6%

PRODUCT INFO                                                                Item: 51

Improved Solubility for Reconstitution over Bacteriostatic Water. 

should be use only with IGF1 and IGF1-LR3 due to PH acidity, ( Peptides are pH sensitive- using Acetic Acid would destroy the peptide, with the exception of IGF1 and IGF1-Lr3 which needs a more acidic pH to remain stable). 

Why use Acetic acid for IGF1 and IGF1 LR3 only : 

1 : Acetic acid will break down any fragments stuck in glass or smaller than you can see, by mixing the lyophilized IGF-1 with acetic acid, the IGF-1 molecules are efficiently detached from the glass and solubilized in the mixture. (gently rotate the bottle and allow the solution to get all fragments then add bacteriostatic water to prevent less irritation for injection ) . 
2 : because it s can extend the shelf life, useful when have to inject small dose and want keep the mix up to 30 days (With bac water you have about 28 days shelf life if refrigerated, With acetic acid you have a 2 year shelf life without refrigeration ). 
Acetic acid can be use for storage, (Mix only the time of use). 
acetic acid damaging tissues even 0.6% better get diluted with 80% part Bacteriostatic Water proportion 
20% part Acetic Acid. 

10 ml glass vial/container. 
Effective dosage for most ranges from 1ml - 2ml for IGF & IGF LR3 only

$25.00
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